LOCUS       40924_21633        5240 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGFtef3-hph DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Sato M, Kurahashi A, Nishibori K, Fujimori F.
  TITLE     Development of a transformation system for the edible mushroom 
            Grifola frondosa: demonstrating heterologous gene expression and 
            RNAi-mediated gene silencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Sato M, Kurahashi A, Nishibori K, Fujimori F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2014) Contact:Masayuki Sato Yukiguni Maitake Co. 
            Ltd., Research and Development; 89 Yokawa, Minamiuonuma, Niigata 
            949-6695, Japan URL :http://www.maitake.co.jp
REFERENCE   3  (bases 1 to 5240)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5240)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-FEB-2014) Contact:Masayuki Sato Yukiguni Maitake Co. Ltd., 
            Research and Development; 89 Yokawa, Minamiuonuma, Niigata 949-6695,
            Japan URL :http://www.maitake.co.jp"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..727
                     /gene="Gf.TEF3"
                     /regulatory_class="promoter"
     gene            1..727
                     /gene="Gf.TEF3"
                     /label=Gf.TEF3
     CDS             728..1750
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      1754..2550
                     /gene="Gf.TEF3"
                     /regulatory_class="terminator"
     gene            1754..2550
                     /gene="Gf.TEF3"
                     /label=Gf.TEF3
     primer_bind     complement(2598..2614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2622..2638)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2646..2676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2691..2712)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3000..3588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3762..4619)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4620..4724)
                     /label=AmpR promoter
     primer_bind     5198..5214
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cactgggtgg aagctaatag caagcattat gaagctggtg gttatgtgcg tgccctttca
       61 tttacgtcga tcgatggcac gtcgcaaaca tgctcaccgg atcccttctg cagcaattca
      121 caccgcttag tttcgctcag ctgaatgaga agcagtgaga tggtaaggcc atagctcgga
      181 catgaatcgg atgtcatgta gttgcgatgc atgtatactc gtagttccga tgtcggatac
      241 ggcgttagca agaagtgctg tcatttgaac gctgcatgat ggcccgtggg gttagcttcc
      301 ccgcattcac atcggatgac tgagagacct ggaggcgccc gaggctgagc tggatgtgac
      361 cagcgtgaca ggacacgtga atacagtgca ttttttcgaa gtccgcctgc ttacaccagc
      421 gttatataaa ccgagactca acaatccgta accttatccc cactctttcc tttcctcttt
      481 tcttctattg tcctatagaa ggtggctttt cttcgtctct gtcatccctt tcaacagccc
      541 gctaactttt tgcgcaggct ccggccaacc tagaaataga gtgctctatc atccttgacg
      601 accttttcgc gtcttatcgc attttacaag taccccgatc atatcatcgt taaaggtgat
      661 taccacgaca cctaatcctc tcacttttgc aggtctgaca atgagatcgg tcgcccagat
      721 attcgaaatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
      781 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
      841 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
      901 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
      961 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     1021 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     1081 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     1141 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     1201 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     1261 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     1321 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     1381 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     1441 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     1501 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     1561 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     1621 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     1681 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     1741 ggcaaaggaa tagacgttgt ccgtcgccgt tgttgtttat cctgtctcta tcgctgtgtt
     1801 gttctatccg gacgtctctc tcggctatat atctacgtct tgtactctgt accctagtat
     1861 tacctctact tcacgcattt atgacatttc gcttctttct gttcgaattg gaggatgata
     1921 cggagctaga cattgagcgt gaagcagtgt cctcgatgca actgcggagc gattgtcggt
     1981 gccacaggcg cgttctgacg gctgcgcgct caccgcaatt attatttgaa tgacggctcc
     2041 ttgaggcttc caatctcttt gcttccaact tccacacttc tggtttcacg acctgcgatg
     2101 cccaatgttc atgaataccc agtgcattcg ttcgagctgt tcgctcgctc cgcgacgaac
     2161 acactcacac cgaacacgac tcagcgaaac acgcttatag tcgctgcttg ctatatcctg
     2221 gccattgcca ttctatggtg cgttccctag cctctgagct tctgcgagtg gcgtcgaatt
     2281 tgactcttct caggcatgtg ccgtatctct ctgcgatcag tgagttttgc aaagttcatt
     2341 gtactttcca atggactaaa cgagttgttt ttttttgcgc tcagtatatc cttttaagtg
     2401 agtacattca gaaaaacgta ataggacggt gaggtctcat tagcatggta ggctattgac
     2461 tgtaggattc catgaggtta gtgcatgttc agctttcgag gtgcttcctg ggcttatgaa
     2521 ccgactgcag atgagccatg caatcgcagg gatcctctag agtcgacctg caggcatgca
     2581 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2641 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2701 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2761 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2821 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2881 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2941 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3001 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3061 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3121 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3181 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3241 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3301 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3361 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3421 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3481 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3541 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3601 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3661 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3721 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3781 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3841 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3901 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3961 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4021 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4081 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4141 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4201 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4261 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4321 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4381 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4441 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4501 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4561 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4621 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4681 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4741 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     4801 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     4861 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     4921 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     4981 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     5041 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     5101 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     5161 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
     5221 gagctcggta cccggggatc
//
